. Author manuscript; available in PMC: 2020 Oct 22.

Published in final edited form as: Biochemistry. 2019 Oct 10;58(42):4293–4303. doi: 10.1021/acs.biochem.9b00719

Table 3.

Estimated relative reaction rates of the Photinus luciferases and variants

	Relative rates (%)^a			K_m (μM) ^b	Relative Φ_BL^c D-LH₂-AMP
Enzyme	Overall	Adenylation	Oxidation	K_m (μM) ^b	Relative Φ_BL^c D-LH₂-AMP
PpyWT	100 ± 10	100 ± 15	100 ± 9	14 ± 3	100 ± 9
PsnWT	155 ± 3	80 ± 10	173 ± 10	24 ± 8	106 ± 3
Pps6	160 ± 8	97 ± 10	182 ± 18	11 ± 4	120 ± 6
Pps7	220 ± 11	117 ± 18	227 ± 16	12 ± 2	115 ± 8

Overall and oxidation rates (expressed relative to PpyWT) were estimated using k_cat values obtained with D-LH₂ and Mg-ATP or D-LH₂-AMP, respectively. Adenylation rates were estimated from the rates of dehydroluciferyl-adenylate (L-AMP formation).

The K_m values were calculated from the oxidation reaction rates.

Determined by measuring the total light emitted from D-LH₂-AMP using an excess of enzyme to insure consumption of substrate.