Figure 8.

Inhibition of SIRT-1 enhances RV-induced IFN responses in normal airway epithelial cells. Normal mucociliary-differentiated cell cultures were pretreated with EX-527 for 24 h, infected with sham or RV and then incubated in the presence or absence of EX-527 for another 24 h. Total RNA was isolated and subjected to qPCR to determine mRNA expression of IFNs (a - c). Protein levels of IFNs in the basolateral medium was assessed by ELISA (d and e). From some experiments, total protein was isolated and subjected to Western blot analysis with total and phospho-STAT1 and STAT2 antibodies (f). Image is representative of 4 independent experiments. Viral load was determined by quantitative RT-qPCR and data represent range with median from 4 experiments (g). Data in a - e represent mean ± SEM calculated from 4 independent experiments and statistical significance was determined by ANOVA.