Figure 9.

Quercetin treatment reduces RV-induced IFN expression via SIRT-1. Mucociliary-differentiated COPD airway epithelial cell cultures were treated with varying concentrations of quercetin for 24 h and SIRT-1 expression and viral endocytosis was determined by Western blot analysis and Flow cytometry respectively (a and b). COPD airway epithelial cell cultures were pretreated with 1 μM quercetin for 24 h, infected with sham or RV, incubated for 24 h in the presence or absence of EX-527and mRNA expression of IFNs was determined. Data was normalized to G3PDH and then expressed as fold increase over sham (c - e). Basolateral medium was analyzed by ELISA to determine the protein levels of IFNs (f and g). Viral load was determined by quantitative RT-qPCR and data represent range with median (h). Data represents mean ± SEM calculated from 4 independent experiments (a – e) and statistical significance analyzed by ANOVA.