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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Insect Physiol. 2019 Aug 22;118:103932. doi: 10.1016/j.jinsphys.2019.103932

Figure 1: Overexpression of myr-AsteAkt-HA in the abdominal fat body suppressed ILP2 but increased ILP3 transcript expression in the head.

Figure 1:

Heads from 3 to 5 day old TG and NTG A. stephensi females were dissected to assess mRNA expression of (A) ILP1, (B) ILP2, (C) ILP3, (D) ILP4, and (E) ILP5, at 0h (non-blood fed, NBF), 6h, 24h, 36h, and 48h post blood meal. ILP expression was quantified by qPCR. NTG samples were used as a control 2for each time points for the relative transcript expression determination. The dotted line on the graphs represents normalized transcript levels in the NTG samples (control). The Y-axis represents the log2 of fold changes which were calculated by the 2^−ΔΔCt method in which the Ct values of TG gene were normalized to the level of NTG control within each time point. Each value is the mean ± SEM of three independent experiments from five pooled mosquito heads. Results were analyzed using Student’s paired t-test (* = p<0.05; Graphpad Prism 7) to determine differences between TG mosquitoes and NTG sibling controls. All mRNA expression studies were replicated a minimum of three times.