TABLE 2.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source |
|---|---|---|
| Strains | ||
| BW25113 | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | 47 |
| K2479 | BW25113 ΔnfsA ΔnfsB | This study |
| K2506 | BW25113 ΔnfsA ΔnfsB ΔahpF | This study |
| K2511 | BW25113 ΔnfsA ΔnfsB ΔahpF pCA24N::ahpF | This study |
| K2526 | BL21 pCA24N::nfsB | This study |
| K2528 | BW25113 ΔahpC pCA24N::ahpF | This study |
| Plasmids | ||
| pCP20 | Apr, Cmr, FLP+, λ cI857+, λ pR Repts; For removal of kan markersby FLP-mediated site-specific recombination | 77 |
| pCA24N::ahpF | Cmr; lacIq, pCA24N PT5-lac::ahpF | 31 |
| pCA24N::nfsB | Cmr; lacIq, pCA24N PT5-lac::nfsB | 31 |