FIG 5.

Smoflipid inhibits the yeast-to-hypha transition. (A) C. albicans strain CAI4+pKE4-GFP was grown in minimal medium alone (control) or minimal medium supplemented with 10% Smoflipid, 10% Intralipid, or 5% Omegaven for 4 h. Wet mounts (20 μl) were prepared, and images of 5 nonadjacent fields were captured by fluorescence microscopy. A representative (n = 3) of each is depicted. Bar, 30 μm. (B) Hyphal cells, as well as total cells, per field were enumerated for 10 random images. Data are representative of the mean (n = 3) percent hyphae per field. Error bars represent SEM. ***, P < 0.001 using a one-way ANOVA and Dunnett’s posttest. (C) Using similar images above, hyphal lengths were quantitatively assessed using ImageJ. Data represent the median (n = 3) hyphal length ± SD. *, P < 0.05 using a one-way ANOVA and Dunnett’s posttest.