FIG 2.

Susceptibility and resistance to itraconazole (ITC), voriconazole (VRC), fluconazole (FLC), ketoconazole (KTC), miconazole (MICO), and cycloheximide (CYH) of S. cerevisiae transformed with different plasmids encoding MFS and ABC transporters. The efflux pumps conferring drug resistance are highlighted in bold. Yeasts were spotted at different dilutions on MMG plates, as described in Materials and Methods. As a control, S. cerevisiae was spotted on plates without antifungal drugs. The plates were incubated at 30°C for 7 days. S. cerevisiae transformed with plasmid pYES2 was used as a control of susceptibility (top row).