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. 2019 Mar 26;28(5):1439–1446. doi: 10.1007/s10068-019-00596-7

Fig. 2.

Fig. 2

Anti-melanogenic activity of EtOH extracts of Rosa gallica petals via suppression of tyrosinase expression/activity. The effect of the extracts on tyrosinase activity was evaluated using mushroom tyrosinase enzyme (NC, negative control; PC, positive control) (A). The effect of the extracts on melanin production was measured. Detailed procedure was described in Materials and methods (B). Tyrosinase expression was measured following treatment of the extracts. Western blot analysis was used to reveal the effect of the extracts on the tyrosinase expression (C). Cytotoxicity of the extracts in B16F10 melanoma cells was estimated. MTS assay was performed to verify non-cytotoxic concentration of the extracts as described in Materials and methods (Cont, untreated control) (D). Data represent 3 independent experiments, which gave similar results. The (##) sign indicates a significant (p < 0.001) increase, and (**) indicates a significant (p < 0.001) decrease