Figure 1.

IFN-λ3 inhibits PEDV infection and is superior to IFN-α in IPEC-J2 cells. (A,B) Inhibition of PEDV infection in IPEC-J2 cells by IFN-λ3 or IFN-α. (A) IPEC-J2 cells were primed with IFN (1,000 ng/ml) for 24 h and infected with PEDV CV777 at an MOI of 1, and the PEDV viral genome numbers (A) and titers (B) at 48 hpi were determined by RT-qPCR and TCID₅₀ titration, respectively. The results are presented as the means ± SEMs (n = 3). The differences between groups were compared using an unpaired Mann-Whitney test. P < 0.05 was considered significant, and the p values are expressed as follows: ^*p < 0.05, ^**p < 0.01, ^***p < 0.005, and ^****p < 0.001. (C) Representative images of inhibition of PEDV infection in IPEC-J2 cells by IFN-λ3 or IFN-α. IPEC-J2 cells were primed with IFN (1,000 ng/ml) for 24 h and infected with PEDV CV777 at an MOI of 1, and the cells were fixed with 4% paraformaldehyde at 48 hpi, and the expression of PEDV N protein was detected with mouse anti-PEDV N mAb (red). DAPI was used to stain cellular nuclei (blue). Bar = 200 μm.