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. 2019 Oct 17;10:2394. doi: 10.3389/fimmu.2019.02394

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Copyright © 2019 Li, Xue, Fu, Yin, Feng and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of PEDV infection by IFN-λ3 selectively elicited pMX2 or pRSAD2. (A–D) The overexpression of pMX2 or pRSAD2 inhibited PEDV infection. pMX2 or pRSAD2 was cloned and expressed in the eukaryotic expression vector pCAGGS-HA. Vero E6 cells were transfected with pMX2 (A,B) or pRSAD2 (C,D) and pCAGGS-HA for 24 h and then were infected with PEDV CV777 (MOI = 1). PEDV infection was determined by measuring PEDV genomes (A,C) and PEDV N protein (B,D). The differences between groups were compared using an unpaired Mann-Whitney test. P < 0.05 was considered significant, and the p values are expressed as follows: ^*p < 0.05, ^**p < 0.01, ^***p < 0.005, and ^****p < 0.001.