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. 2019 Oct 17;10:2408. doi: 10.3389/fimmu.2019.02408

Figure 2.

Figure 2

Neural apoptosis in the peri-infarct area at 24 h after MCAO/R with or without PTE administration. (A) Immunofluorescence double-staining of TUNEL (green) and DAPI (blue) was performed on frozen sections fixed at 24 h after MCAO/R or sham surgery. Scale bars = 100 μm. (B) The percentage of TUNEL-positive cells in the peri-infarct area was counted under fluorescence microscopy as an average of five visual fields. (C) The neuronal apoptotic rate was assessed using immunofluorescence double-staining of apoptotic cells (TUNEL, green) and neurons (NeuN, red) under the same conditions. Scale bars = 50 μm. (D) The percentage of TUNEL-positive neurons in the peri-infarct area was counted under fluorescence microscopy as an average of five random visual fields. Values are expressed as mean ± standard deviation (n = 8). ap < 0.01, compared with Sham + Vehicle. bp < 0.01, compared with MCAO/R + Vehicle. Significance was determined using a one-way analysis of variance. MCAO/R, middle cerebral artery occlusion and reperfusion; DAPI, 4′,6-diamidino-2-phenylindole; PTE-5/10, pterostilbene 5 or 10 mg/kg; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling.