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. 2019 Oct 17;9:356. doi: 10.3389/fcimb.2019.00356

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Copyright © 2019 Chen, Rathod, Chiu, Chen, Tsai and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth kinetics of C. difficile R20291 and 630Δerm lrp strains. 12-h cultures of the parental strains, lrp mutant strains, and complemented strains were used for inoculation into fresh BHIS (nutrient rich) (A,B) and CDMM (nutrient poor) (C,D) medium. The OD600 values were recorded at fixed time points. Data are represented as the mean ± standard error of the mean, and the results are representative of at least three independent experiments.