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. 2019 Oct 23;10:4834. doi: 10.1038/s41467-019-12772-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Chromosomal instability in tetraploid embryos is not attributable to supernumerary centrosomes. a, b, d and e Representative time-lapse images and illustrations of live 8-cell control (a), 4-cell binucleated (b), 16-cell control (d) and 8-cell tetraploid embryos (e) labelled with SiR Tubulin (grey) and co-expressing H2B:RFP (cyan) and CDK5RAP2:GFP (magenta and inverted grey). A major microtubule organising centre (MTOC) can be observed in the 8-cell control, 16-cell control and 8-cell tetraploid embryo during interphase (red arrows) and a newly assembled MTOC can be observed during mitosis (blue arrows). In binucleated embryos, two major MTOCs (red arrows) can be observed during interphase and two newly assembled MTOCs (blue arrows) can be observed during mitosis. c Measurements of the angle between the two individualised spindles during the binucleated division. Line colours represent the different types of chromosome segregation events associated with a specific cell division (n = 23 divisions from 12 embryos). Chromosome segregation errors observed included: lagging chromosomes resulting in micronuclei formation (lagging with MN); lagging chromosomes that did not result in micronuclei formation (lagging without MN); and chromosome bridges. Scale bars = 10 µm. NEBD nuclear envelope breakdown