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. 2019 Oct 23;9:15225. doi: 10.1038/s41598-019-51679-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibody responses against the mixture of L1 + L2 peptides as an antigen in different groups: (A) total IgG, (B) IgG1, (C) IgG2a and (D) IgG2b; Mice sera were prepared from the whole blood samples of each group (n = 6) two weeks after the last immunization. All analyses were performed in duplicate for each sample shown as mean absorbance at 450 nm ± SD.