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Immunization program for in vivo analysis.
| Groups | First injection or priming (Day 0) | Second injection or first booster (Day 14) | Third injection or second booster (Day 28) | Challenge with C3 cells (Two weeks after second booster) |
|---|---|---|---|---|
| G1 | pcDNA-L1 | pcDNA-L1 | pcDNA-L1 | C3 |
| G2 | pcDNA-L2 | pcDNA-L2 | pcDNA-L2 | C3 |
| G3 | pcDNA-L1 + pcDNA-L2 | pcDNA-L1 + pcDNA-L2 | pcDNA-L1 + pcDNA-L2 | C3 |
| G4 (Control) | pcDNA3.1 (empty vector) | pcDNA3.1 (empty vector) | pcDNA3.1 (empty vector) | C3 |
| G5 (Control) | PBS | PBS | PBS | C3 |
