Table 4.
The selected CTL epitopes of HPV L1 protein based on binding affinity.
| Protein Name | Position | Epitope Sequence | No. of Alleles | Top alleles | NetMHCpan Average Rank Scores* | ProPred-I Average Scores** | Syfpeithi Average Scores** | Conservancy (=>75%) | Allergenicity |
|---|---|---|---|---|---|---|---|---|---|
| L1 | 12–21 (Type 16) | YLPPVPVSKV | 17 |
HLA-A02:01 HLA-A03:01 HLA-B07:02 |
0.574 | 97.324 | 17.285 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen |
| 12–21 (Type 18) | YLPPPSVARV | 17 |
HLA-A02:01 HLA-A03:01 HLA-A26:01 |
0.616 | 96.521 | 16.818 |
Type 45: 100% Type 39: 90% Type 59: 90% Type 68: 90% |
Non-allergen | |
| 114–125 (Type 16) | GVGISGHPLLNK | 13 |
HLA-A03:01 HLA-B58:01 |
0.911 | 61.956 | 13.673 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 52: 100% Type 51: 83.33% Type 58: 83.33% |
Non-allergen | |
| 104–115 (Type 18) | GVEIGRGQPLGV | 12 |
HLA-B08:01 HLA-B39:01 HLA-B40:01 |
0.831 | 64.810 | 13.678 |
Type 59: 100% Type 68: 100% Type 39: 91.67% Type 45: 91.67% Type 51: 91.67% Type 58: 91.67% Type 56: 75% |
Non-allergen | |
| 114–125 (Type 18) | GVGLSGHPFYNK | 11 |
HLA-A01:01 HLA-A03:01 HLA-B58:01 |
0.840 | 63.982 | 14.129 |
Type 45: 91.67% Type 59: 91.67% Type 51: 83.33% Type 68: 83.33% |
Non-allergen | |
| 411–421 (Type 16) | GGTLEDTYRFV | 14 |
HLA-A01:01 HLA-A02:01 HLA-A03:01 |
0.789 | 68.232 | 14.750 |
Type 31: 81.82% Type 33: 63.64% Type 35: 81.82% |
Non-allergen | |
| 414–425 (Type 18) | PTTSLVDTYRFV | 14 |
HLA-A01:01 HLA-A02:01 HLA-A03:01 |
0.850 | 75.541 | 15.868 |
Type 45: 100% Type 59: 91.67% Type 33: 75% Type 52: 75% Type 58: 75% |
Non-allergen | |
| 460–470 (Type 16) | DQFPLGRKFLL | 16 |
HLA-B08:01 HLA-B07:02 HLA-A24:02 HLA-A26:01 HLA-B39:01 |
0.757 | 79.497 | 16.523 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 39: 100% Type 52: 100% Type 58: 100% Type 59: 100% Type 68: 100% Type 51: 90.91% Type 56: 90.91% Type 18: 81.82% Type 45: 81.82% |
Non-allergen | |
| 461–471 (Type 18) | DQYPLGRKFLV | 17 |
HLA-B08:01 HLA-B07:02 HLA-A24:02 HLA-A26:01 HLA-B39:01 |
0.723 | 129.350 | 16.531 |
Type 45: 100% Type 16: 81.82% Type 31: 81.82% Type 33: 81.82% Type 35: 81.82% Type 39: 81.82% Type 52: 81.82% Type 56: 81.82% Type 58: 81.82% Type 59: 81.82% Type 68: 81.82% |
Non-allergen |
*lower rates show better binding affinity, **Higher rates show better binding affinity.