Table 5.
The selected CTL epitopes of HPV L2 Protein, based on binding affinity.
| Protein Name | Position | Epitope Sequence | No. of Alleles | Top alleles | NetMHCpan Average Rank Scores* | ProPred-I Average Scores** | Syfpeithi Average Scores** | Conservancy (= > 75%) | Allergenicity |
|---|---|---|---|---|---|---|---|---|---|
| L2 | 11–20 (Type 16) | KRASATQLYK | 17 |
HLA-A03:01 HLA-B58:01 HLA-B15:01 HLA-B27:05 |
0.576 | 413.070 | 17.280 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen |
| 11–20 (Type 18) | KRASVTDLYK | 17 |
HLA-A03:01 HLA-B58:01 HLA-B15:01 HLA-B27:05 |
0.626 | 415.190 | 17.360 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen | |
| 60–71 (Type 18) | GTGSGTGGRTGY | 13 |
HLA-A03:01 HLA-A01:01 HLA-B15:01 |
1.502 | 33.7167 | 11.585 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen | |
| 280–291 (Type 16) | DPDFLDIVALHR | 12 |
HLA-A01:01 HLA-A03:01 HLA-A26:01 |
1.198 | 53.281 | 15.375 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen | |
| 293–303 (Type 16) | ALTSRRTGIRY | 10 |
HLA-A01:01 HLA-A15:01 HLA-A26:01 |
0.873 | 42.308 | 14.720 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen | |
| 273–284 (Type 18) | DSDFMDIIRLHR | 12 |
HLA-A40:01 HLA-A01:01 HLA-A03:01 |
1.031 | 65.570 | 14.754 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen | |
| 286–296 (Type 18) | ALTSRRGTVRF | 10 |
HLA-A58:01 HLA-B27:05 HLA-B08:01 |
0.845 | 43.607 | 14.590 |
Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% |
Non-allergen |
*lower rates show better binding affinity, **Higher rates show better binding affinity.