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. 2019 Oct 23;9:15202. doi: 10.1038/s41598-019-50917-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mit-A - a bona fide anti-CSC target. (A,C,E) HT29, HCT116 and KM12 cells, respectively, were plated in 96 well ultra-low attachment plates and treated with indicated doses of Mit-A for 5 consecutive days to allow the formation of spheres. Images were taken through an inverted microscope and area of spheres was counted (B,D,F, respectively) by using ImageJ software. (G) Area of the spheres treated with different anti-cancer drugs, Carboplatin, Oxaliplatin, 5-FU or Mit-A. (H,I) Total RNA was isolated from HT29 (H) and HCT116 (I) spheres treated with vehicle and Mit-A with indicated dose, and OCT-4 gene expression analyzed by qPCR. Each data point indicates mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. A representative of two experiments is shown.