Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 23;9:15246. doi: 10.1038/s41598-019-51608-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Basic principle of Pluronic (PF-127) gel-based burrowing assay. (a) The burrowing assay is conducted in a 12-well plate; in each well, animals are stimulated to move through a gel towards a food source placed at the top. The inset shows the nematodes that have successfully burrowed and reached the food source (E. coli) at the top of the gel (See also supplementary video S1). (b) Burrowing performance of day 1 wild-type adults in the presence and absence of E. coli bacteria in 26% w/w PF-F127. Gel thickness, H = 0.9 cm. N = 39 and 35 animals in the presence and absence of bacteria, respectively. (c) Calcium imaging shows the muscle contractions as the nematode is burrowing in the PF-127 gel. Strain is HBR4: goeIs3 HBR4: goeIs3[Pmyo-3::GCaMP3.35::unc-54-3′utr, unc-119] expressing the calcium indicator GCaMP3 in body wall muscles. Images are taken with 5 s intervals. Arrow heads point to the tail that appears faded in i and ii due to the 3D locomotion. In all three images, the head is on the left. (d) Dynamic quantification of calcium signaling activity from an animal during a 10 second burrowing episode. Each pixel is 1 second apart on the x-axis.