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. 2019 Oct 17;10:1277. doi: 10.3389/fpls.2019.01277

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Copyright © 2019 Liu, Liu, Wang, Chen, Fan, Wu and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of ZMBJ-CMV201-2b_N81-STTM. (A) Schematic ZMBJ-CMV201-2b_N81 vector and insertion of STTM. STTM fragments are inserted at the multiple cloning sites (MCS). (B) One-step method used to construct a STTM sequence and the recombination-based ligation method. The STTM fragments were PCR amplified with specific forward and reverse primers ( Supplementary Table S1 ). Bold, the two adapter sequences; Red, complementary strand of miR165/166; Italicized, a three nucleotide interruption linker sequence; Blue, 48 nt template; Arrows indicate the locations of the forward and reverse primers.