Skip to main content
. 2019 Oct 17;10:1277. doi: 10.3389/fpls.2019.01277

Figure 4.

Figure 4

Silencing miR167 expression in maize using ZMBJ-CMV-STTM167. (A) A diagram of the STTM167 insert in the ZMBJ-CMV-based VbMS vector. 48 nt, a 48-nt long stem-loop like linker; —, no nt at this position. (B) Roots of maize seedlings infected with ZMBJ-CMV (control) or ZMBJ-CMV-STTM167 were photographed at 14 dpi. Bars = 2 cm. (C) Measurement of total root length of the ZMBJ-CMV or ZMBJ-CMV-STTM167 infected maize seedlings at 14 dpi. ***, p < 0.001. (D) Relative expression of miR167 in the ZMBJ-CMV or ZMBJ-CMV-STTM167 infected maize plants determined by stem-loop RT-qPCR. (E) Relative expression of miR167 target genes ZmARF3, ZmARF9, ZmARF16, ZmARF18, ZmARF22, ZmARF30, ZmARF34 and ZmIAR3 in the ZMBJ-CMV or ZMBJ-CMV-STTM167 infected maize seedlings. The first true leaves of the assayed plants were harvested at 14 dpi and used for this study. The results were analyzed and presented as described in Figure 2 . *, p < 0.05; ***, p < 0.001.