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. 2019 Oct 23;9:15218. doi: 10.1038/s41598-019-51532-y

Figure 3.

Figure 3

Granule cell precursors retract all processes before cell division. (AE) Examples of typical cellular morphologies of GCPs at different stages of mitosis, characterised by distinctive PH3 condensation pattern (red). White dots delineate the cell morphology on merged images. (A) A cell in prophase with an example of a short, extended process. Prophase cells typically have even longer processes. (B) A cell in pro-metaphase with processes that appear to be retracting. (C) A cell in metaphase with very short processes. (D) A cell in anaphase with no processes visible. (E) A cell in late telophase. There are no processes extended at this point. (F) Proportions of cells with an extended process at different stages of mitosis. As the mitotic cell approaches anaphase and telophase, it is increasingly less likely to be extending a process (n = 154 cells from 3 cerebella). (G) Stills of five different GCPs captured minutes prior to cytokinesis during time-lapse imaging of cerebellar organotypic slices. The cells (red arrows) have no processes and a completely round morphology. All observed cells in time-lapse imaging underwent this stage of morphological transition before division (n = 16). Scale bar = 5 μm.