Table 2.
Characteristics of the 11 included cell studies.
| Study (years) | Appellation (n = experimental/control group) | Organism age tissue | Primary cells or subcultured cells | Model (method) | Treatment group (method to astragal sides) | Control group | Outcome index (time) | Intergroup differences |
|---|---|---|---|---|---|---|---|---|
| Zhou et al., 2016 | RCM (6/6) | WistarSuckling miceMyocardium | Primary cells | Received H2O2 (50 μmol/L) | NGR1 (10 μmol/L; 24 h) before molding | No treatment | 1. LDH 2. SOD 3. MDA 4. Cell viability 5. Apoptosis rate 6. p-ERK1/2 7. ERK1/2 8. p-p38 9. p38 | 1. P < 0.01 2. P < 0.01 3. P < 0.01 4. P < 0.01 5. P < 0.01 6. P < 0.01 7. P < 0.01 8. P < 0.01 9. P < 0.01 |
| Zhou et al., 2017 | RCM (6/6) | WistarSuckling miceMyocardium | Primary cells | Received H2O2 (50 μmol/L) | NGR1 (10 μmol/L; 24 h) before molding | No treatment | 1. LDH 2. SOD 3. MDA 4. Cell viability 5. Apoptosis rate | 1. P < 0.01 2. P < 0.01 3. P < 0.01 4. P < 0.01 5. P < 0.01 |
| Wan et al., 2015 | RCM (6/6) | WistarSuckling miceMyocardium | Primary cells | Received H2O2 (1 mmol/L) | Received NGR1 (100 μmol/L; 24 h) before molding | No treatment | 1. Cell viability 2. Apoptosis rate 3. MDA 4. SOD5. p-JNK 6. Bax 7. Bcl -2 | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 7. P < 0.05 |
| Yu et al., 2016 | H9C2 (6/6) | Rat embryonic cardiomyoblast- derived H9c2 cardiomyocytes | Subcultured cells | H/R (6 h/12 h) | NGR1 (20 μmol/L; 24 h) before molding | No treatment | 1. Cell viability2. Extracellular LDH3. ROS4. Relative intensity of red/green fluorescence5. PIP positive cell rate6. TUNEL-positive7. GRP78/β-actin8. P-PERK/PERK9. P- eIf2α/eIf2α10. IRE1/β-actin11. ATF6/β-actin | 1. P < 0.001 2. P < 0.001 3. P < 0.01 4. P < 0.001 5. P < 0.01 6. P < 0.001 7. P < 0.01 8. P < 0.001 9. P < 0.001 10. P < 0.01 11. P < 0.001 |
| He et al., 2014 | H9C2 (6/6) | A rat cardiac myoblast cell line | Subcultured cells | OGD/R(15 h) | NGR1 (100 μmol/L) |
No treatment | 1. TUNEL-positive2. Cell viability3. LDH4. Bcl-2/Bax5. Cleaved caspase-3/procaspase-36. ATP7. AMP8. ATP synthase activity9. P-AMPK/β-actin10. ATPsynthase-α/β-actin11. ATP synthase-β/β-actin12. ATP 5D/β-actin13. ROCK/β-actin14. P-MYPT1/MYPT1 | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 7. P < 0.05 8. P < 0.05 9. P < 0.05 10. P > 0.05 11. P > 0.05 12. P < 0.05 13. P < 0.05 14. P < 0.05 |
| Liu et al., 2019 | RCM | NeonatalSD rats Myocardium | Primary cells | OGD (6 h) | NGR1 (20 μmol/L) for 24 h | No treatment | 1. Cell viability2. Apoptotic cells3. RNA level expression of miR-214. mRNA and protein levels of PTEN | 1. P < 0.05 2. P < 0.05 3. P < 0.05 P < 0.05 |
| Meng et al., 2014 | Primary cortical neurons (6/6) | SD rats embryo cerebral cortices | Primary cells | OGD/R (2 h/24 h) | NGR1 (25 μM) for 24 h before ischemia | Treated with DMSO (final concentration was 0.1%) | 1. Intracellular ROS2. NADPH oxidase activity3. Superoxide levels4. Mitochondrial superoxide5. MDA6. Protein carbonyl7. 8-OHdG8. TUNEL-positive cells rate9. Apoptosis rate10. Ratio of red to green fluorescence intensity11.Cell viability12. LDH13. Caspase-3 activity | 1. P < 0.01 2. P < 0.01 3. P < 0.01 4. P < 0.01 5. P < 0.01 6. P < 0.01 7. P < 0.01 8. P < 0.01 9. P < 0.01 10. P < 0.01 11. P < 0.01 12. P < 0.01 13. P < 0.01 |
| Wang, 2016 | Primary cortical neurons (5/5) | SD rats embryo cerebral cortices | Primary cells | OGD/R (1.5 h/24 h) | NGR1 (10 μmol/L) | DMSO (1%) | 1. Cell viability2. LDH3. Ratio of GRP78/β-actin4. Ratio of P-PERK/PERK5. Cleaved-caspase-12/caspase-126. Ratio of P-IRE1α/IRE1α7. Ratio of BCL-2/β-actin | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 7. P < 0.05 |
| Hou et al., 2017 | Primary cortical neurons (5/5) | SD rats embryo cerebral cortices | Primary cells | OGD/R (1.5 h/24 h) | NGR1 (10 μmol/L) | 1. Cell viability2. LDH3. ATF6/Akt4. P-Akt/Akt5. Cleaved Caspase-3/β-actin6. Bax/β-actin | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 | |
| Wang et al., 2017 | Primary cortical neuron (5/5) | SD rats embryo cerebral cortices | Primary cells | OGD/R (1.5 h/24 h) | NGR1 (10 μmol/L) | DMSO (1%) | 1. Cell viability2. Ratio of p-PLCβ/PLCβ3. Ratio of p-PLCγ/PLCγ4. Ratio of IP3R1/β-actin5. Ratio of p-PERK/β-actin6. Ratio of p-IRE1/β-actin7. Ratio of CHOP/β-actin8. Ratio of p-CaMKII/β-actin9. Ratio of p-P38/β-actin10. Ratio of p-JNK/β-actin11. Ratio of TUNEL-positive cells | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 7. P < 0.05 8. P < 0.05 9. P < 0.05 10. P < 0.05 11. P < 0.05 |
| Tu et al., 2018 | Primary cortical neuron (5/5) | Rats embryo cerebral cortices | Primary cells | OGD/R (1.5 h/24 h) | NGR1 (10 μmol/L) | 1. Cell viability2. LDH3. Ratio of PI3K/β-actin4. P-Akt/T-Akt5. P-mTOR/T-mTOR6. P-P70S6K/P70S6K7. P-4EBP-1/4EBP-18. P-JNK/T-JNK9. P-c-JUN/c-JUN | 1. P < 0.05 2. P < 0.05 3. P < 0.05 4. P < 0.05 5. P < 0.05 6. P < 0.05 7. P < 0.05 8. P < 0.05 9. P < 0.05 |
RCM, rat cardiac myocytes; OGD/R, oxygen glucose deprivation/reoxygenation; LDH, lactic dehydrogenase; SOD, superoxide dismutase; MDA, TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling; p-ERK1/2, phospho extracellular regulated protein kinases; p-JNK, phospho c-Jun N-terminal kinase; ERK1/2, extracellular regulated protein kinase; Bax, Bcl-2 Associated X protein; Bcl-2, B-cell lymphoma-2; ROS, reactive oxygen species; GRP78, glucose-regulated protein 78; P-PERK, phospho protein kinase RNA-like endoplasmic reticulum kinase; PERK, PKR-like endoplasmic reticular kinase; P-eIf2α, phospho-eukaryotic initiation factor 2α; eIf2α, eukaryotic initiation factor 2; IRE1, inositol-requiring enzyme-1α; ATF6, activating transcription factor 6; P-AMPK, phosphorylation of AMP-activated protein kinase; ROCK, Rho-associated kinase protein; P-MYPT1, phosphorylating myosin light chain phosphatase; MYPT1, myosin light chain phosphatase; ATP, adenosine triphosphate; AMP, adenosine monophosphate; SD rats, Sprague-Dawley; DMSO, dimethyl sulfoxide; OGD, oxygen-glucose deprivation; NADPH, nicotinamide adenine dinucleotide phosphate; 8-OHdG, 8-hydroxy-2’-deoxyguanosine; P-IRE1α, phosphoinositol requiring enzyme 1α; Akt, protein kinase B; P-Akt, phosphorylated Akt; p-PLCβ, phospho phospholipase Cβ; PLCβ, phospholipase C β; p-PLCγ, phospho phospholipase Cγ; PLCγ, phospholipase Cγ; IP3R1, inositol 1;4;5-triphosphate receptor type 1; p-IRE1, inositol-requiring enzyme 1; CHOP, C/EBP homologous protein; p-CaMKII, phospho Ca2+/calmodulin-dependent protein kinase II; PI3K, phosphoinositide 3-kinase; P-mTOR, phosphorylated mammalian target of rapamycin; T-mTOR, mammalian target of rapamycin; P-P70S6K, P70S6K P-4EBP-1, phosphorylated 4E-binding protein 1; 4EBP-1, 4E-binding protein 1.