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. 2019 Oct 17;10:939. doi: 10.3389/fgene.2019.00939

Figure 1.

Figure 1

EBV-miR-BART7-3p induces stem-like properties in EBV-positive NPC cells. (A) Flow cytometric profiles of SP cells among the CNE2 and 5-8F NPC cell lines after stable expression of BART7-3p and incubated with Hoechst 33342. SP cell profiles in the presence of verapamil are shown in the bottom panels. The percentages of SP cells are indicated. (B) Flow cytometric profiles of SP cells within the EBV+HONE1and EBV+HK1 cell populations after treatment of anti-C or anti-miR and incubated with Hoechst 33342. The percentages of SP cells are indicated. (C) Images showing tumor sphere formation in CNE2 and 5-8F NPC cells expressing EBV-miR-BART7-3p (Magnification, ×20; scale bar, 500 um). Columns in chart show number of spheres counted in 9 fields for each group; 0, P < v0.05. (D) Western blot analysis shows up-regulated expression of the stem cell markers ABCG2, OCT4, Nanog, SOX2 at protein level (*P < 0.05, **P < 0.01, ***P < 0.001).