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. 2019 Oct 17;10:939. doi: 10.3389/fgene.2019.00939

Figure 3.

Figure 3

EBV-miR-BART7-3p induces stem-like properties in EBV-positive NPC cells. (A) CNE2 and 5-8F cells were cotransfected with EBV-miR-BART7-3p mimic or NC respectively, and luciferase reporters carrying either the predicted microRNA target site in SMAD7 3′UTR (wt) or its corresponding mutant (mut). Inhibition of EBV-miR-BART7-3p by anti-miR or anti-C was also performed. (B) Western blot analysis of endogenous SMAD7 protein expression levels in 5-8F and CNE2 cells treated with EBV-miR-BART7-3p or NC. Inhibition of EBV-miR-BART7-3p by anti-miR or anti-C was also performed. GAPDH served as the internal control. (C) SMAD7 expression was evaluated using immunohistochemistry assay in tumor tissues derived from mouse models and the control models (Magnification, ×400; scale bar, 25 um). (D) The levels of SMAD7 were detected by qPCR in NPC and NP tissue specimens. (E) SMAD7 mRNA expression was detected by qPCR in NPC samples (with clinical stage). (F) The levels of EBV-miR-BART7-3p and SMAD7 were detected by qPCR in NPC tissue specimens, normalized to the U6 snRNA and GAPDH, respectively. The correlation between EBV-miR-BART7-3p and SMAD7 expression levels was calculated (**P < 0.01, ***P < 0.001).