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. 2019 Oct 17;10:939. doi: 10.3389/fgene.2019.00939

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Copyright © 2019 Cai, Long, Chong, Cai, Tsang, Zhou, Lin, Ding, Zhou, Zhao, Chen, Wang, Lyu, Cho and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of SMAD7 mimicked the EBV-miR-BART7-3p-induced phenotype. (A, B) CNE2 and 5-8F cells treated with siRNAs (si-1, si-2, si-3) targeting SMAD7 were analyzed by Western blot and qPCR. GAPDH served as a loading control. (C) The expression of p-SMAD2, p-SMAD3 and stem-cell markers (NANOG, OCT4) were determined by Western blot in CNE2 and 5-8F cells treated with si-1 and si-2 against SMAD7 or si-NC. GAPDH was internal control. (D) Images showing tumor sphere formation in either si-SMAD7 or si-NC NPC cells (Magnification, ×20; scale bar, 500 um). Columns in chart show number of spheres counted in 9 fields for each group. (*P < 0.05, **P < 0.01, ***P < 0.001).