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. 2019 Oct 17;10:1120. doi: 10.3389/fpls.2019.01120

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Copyright © 2019 Punja, Collyer, Scott, Lung, Holmes and Sutton

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessing the extent of colonization of endophytic fungi inoculated onto exposed stem surfaces of Cannabis sativa. (A) Cut surface of stem following pruning. These cuts were made at the top of the plant, 65 cm from the crown region. (B) Inoculation method used to assess extent of colonization by placing a mycelial plug at the end of the cut stem. (C) Recovery of Penicillium olsonii from colonized stem. (D) Recovery of Fusarium oxysporum from colonized stem. (E) Recovery of Chaetomium globosum from colonized stem. (F) Recovery of Trichoderma harzianum from colonized stem. (G) Distance travelled downward through stem 7 days following inoculation with the fungi shown in (C–F). All tissues were surface-sterilized prior to plating onto PDA+S. Data are from eight replications in each of two experiments. Bars show standard errors.