. 2019 Oct 8;28(5):1297–1305. doi: 10.1007/s10068-019-00691-9

Table 2.

Gene expression related to toxin production by Bacillus probiotics

Target gene	Sequence (5′ → 3′)	References
bceT ^a (Enterotoxin T)	TTACATTACCAGGACGTGCTT TGTTTGTGATTGTAATTCAGG	Matarante et al. (2004)
ces ^b (cereulide)	GGTGACACATTATCATATAAGGTG GTTTTCTGGTAACAGCGTTCTAC	Kim et al. (2015)
cytK ^a (Cytotoxin K)	ACAGATATCGG(GT)CAAAATGC TCCAACCCAGTT(AT)(GC)CAGTTC	Kim et al. (2015)
entFM ^a (Enterotoxin FM)	ATGAAAAAAGTAATTTGCAGG TTAGTATGCTTTTGTGTAACC	Matarante et al., 2004
hblC ^a (L2 subunit of HBL)	GATACTCAATGTGGCAACTGC TTGAGACTGCTCGTCTAGTTG	Chon et al. (2012)
hblD ^a (L1 subunit of HBL)	ACCGGTAACACTATTCATGC GAGTCCATATGCTTAGATGC	Chon et al. (2012)
hblA ^a (B subunit of HBL)	AAGCAATGGAATACAATGGG AGAATCTAAATCATGCCACTGC	Chon et al. (2012)
hblD/A ^a (hemolysin BL enterotoxin)	GGAGCGGTCGTTATTGTTGT GCCGTATCTCCATTGTTCGT	Matarante et al. (2004)
nheA ^a (A subunit of NHE^b)	GTTAGGATCACAATCACCGC ACGAATGTAATTTGAGTCGC	Guinebretiere et al. (2002)
nheB ^a (B subunit of NHE^b)	CTATCAGCACTTATGGCAG ACTCCTAGCGGTGTTCC	Matarante et al. (2004)
nheC ^a (C subunit of NHE^b)	TGGATTCCAAGATGTAACG ATTACGACTTCTGCTTGTGC	Chon et al. (2012)
nprA	GTATACGGAGATGGTGATGG GGATCACTCATAGAGCGAAG	Hisieh et al. (1999)

Target gene

Sequence (5′ → 3′)

References

bceT ^a

(Enterotoxin T)

TTACATTACCAGGACGTGCTT

TGTTTGTGATTGTAATTCAGG

Matarante et al. (2004)

ces ^b

(cereulide)

GGTGACACATTATCATATAAGGTG

GTTTTCTGGTAACAGCGTTCTAC

Kim et al. (2015)

cytK ^a

(Cytotoxin K)

ACAGATATCGG(GT)CAAAATGC

TCCAACCCAGTT(AT)(GC)CAGTTC

Kim et al. (2015)

entFM ^a

(Enterotoxin FM)

ATGAAAAAAGTAATTTGCAGG

TTAGTATGCTTTTGTGTAACC

Matarante et al., 2004

hblC ^a

(L2 subunit of HBL)

GATACTCAATGTGGCAACTGC

TTGAGACTGCTCGTCTAGTTG

Chon et al. (2012)

hblD ^a

(L1 subunit of HBL)

ACCGGTAACACTATTCATGC

GAGTCCATATGCTTAGATGC

Chon et al. (2012)

hblA ^a

(B subunit of HBL)

AAGCAATGGAATACAATGGG

AGAATCTAAATCATGCCACTGC

Chon et al. (2012)

hblD/A ^a

(hemolysin BL enterotoxin)

GGAGCGGTCGTTATTGTTGT

GCCGTATCTCCATTGTTCGT

Matarante et al. (2004)

nheA ^a

(A subunit of NHE^b)

GTTAGGATCACAATCACCGC

ACGAATGTAATTTGAGTCGC

Guinebretiere et al. (2002)

nheB ^a

(B subunit of NHE^b)

CTATCAGCACTTATGGCAG

ACTCCTAGCGGTGTTCC

Matarante et al. (2004)

nheC ^a

(C subunit of NHE^b)

TGGATTCCAAGATGTAACG

ATTACGACTTCTGCTTGTGC

Chon et al. (2012)

nprA

GTATACGGAGATGGTGATGG

GGATCACTCATAGAGCGAAG

Hisieh et al. (1999)

HBL Hemolysin BL, NHE nonhemolytic enterotoxin

^aEnterotoxin genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, bceT, entFM)

^bEmetogenic toxin (ces)