Figure 3.

Impact of cannabis smoke exposure on epithelial cell barrier function and transcriptomic profile. Genes involved in (A) airway epithelial repair and remodeling or epithelial junctions were curated from the RNA‐sequencing dataset performed at 10% CSE or TSE. The expression for each gene is presented for all experimental replicates, with expression for each replicate being scaled by the gene. Calu‐3 cells were exposed to increasing concentrations of CSE (orange) or TSE (blue) (control, 0.625%, 1.25%, 2.5%, 5%, 10%, and 20%) for 24h with outcome measurements of (B) cell viability assessed by lactate dehydrogenase (LDH) assay, (C) transepithelial electrical resistance – TEER (ohms*cm²), (D) transforming growth factor‐alpha (TGF‐α) (pg/mL), and (E) platelet derived growth factor‐AA (PDGF‐AA) (pg/mL). *=P < 0.05 relative to control untreated ‐ Tukey HSD. Error bars represent standard deviation (n = 4).