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. 2019 Jul 26;8(11):1180–1191. doi: 10.1002/sctm.19-0054

Figure 1.

Figure 1

OCT4 mediated direct conversion of human peripheral blood samples to neural precursor cells. (A): Schematic for separation and elution of CD34+ mononuclear cells (MNCs). (B): Flow cytometry plots of MNCs from donor 1 prior (top row) to and after isolation and expansion of CD34+ population (bottom row). (C): Phase contrast images of two example iNPC colonies 10 days after OCT4 transduction (top row) and after colony picking and expansion (bottom row). Scale bar represents 300 μM. (D): Validation of induced neural progenitor cells (iNPCs) after 12 weeks of initial OCT4 infections of CD34+ cells. Derived iNPCs were plated in 24‐well plates (100K seeding) and were fixed and stained for PAX6, Nestin, and SOX2 in combination with staining for DNA (Hoechst). Scale bar represents 50 μM. (E): Evaluation of iNPC expansion over multiple passages and in multiple culture conditions. The potential yield of iNPCs over multiple passages and in multiple conditions (medium and substrate) was calculated. (F): After eight passages, iNPCs yielded 25× greater cell number in culture conditions developed in house, in condition 3.