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Different culture conditions tested for iNPCs expansion
| Condition 1 | Condition 2 | Condition 3 |
|---|---|---|
| RHB‐A basal medium | DMEM/F12 basal medium | DMEM/F12 basal medium |
| N2 (1×) | N2 (1×) | N2 (1×) |
| B27 (1×) | B27 (1×) | B27 (1×) |
| bFGF (10 ng/ml) | bFGF (20 ng/ml) | bFGF (20 ng/ml) |
| EGF (10 ng/ml) | EGF (10 ng/ml) | EGF (10 ng/ml) |
| Poly‐l‐ornithine‐laminin coated plates | Poly‐l‐ornithine‐laminin coated plates | Matrigel coated plates |
