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. 2019 Oct 17;10:1188. doi: 10.3389/fphar.2019.01188

Figure 7.

Figure 7

Phil decreases LPS-induced osteoclast-related cytokine expression in calvarias and in cultured osteoblasts. (A) The calvarias from in vivo experiments were homogenized and the expressions of RANKL and TNFα were determined by real-time PCR. The mRNA levels of these two genes were normalized to GAPDH (n = 3). ###P<0.001 relative to the sham group; **P<0.01 and ***P<0.001 relative to the vehicle group. (B) The hFOB 1.19 cells were treated with LPS (100ng/ml) and different doses of Phil (0, 5, 10, and 20µM) for 24h. The mRNA level of RANKL was determined by real-time PCR and normalized to β-actin (n = 3). ###P<0.001 relative to RANKL-untreated, Phil-untreated group; **P<0.01 and ***P<0.001 relative to RANKL-induced, Phil-untreated group.