Figure 4.

Mst1/2 inhibition using XMU‐MP‐1 increases NRCM proliferation. (a) Representative images of NRCM stained with α‐actinin (red), Ki‐67 (green), and DAPI (blue). NRCM were treated with XMU‐MP‐1 (1–3 μM) for 24 hr prior to fixation and immunostaining. (b) Analysis of Ki‐67 positive cells indicated that treatment with XMU‐MP‐1 at a dose of 3 μM significantly increased the number cardiomyocytes expressing Ki‐67. N = 9; scale bar = 100 μm. Data shown are individual values with means ±SEM. ^* P < .05; one‐way ANOVA with Tukey's multiple comparisons test