Figure 2.

Immunohistochemistry and double-labeling immunofluorescence indicate the increased expression of LPAR1 protein and extent of ERK1/2 phosphorylation in the spinal cord tissue of mice with BCP on day 21: (a) immunohistochemical staining of LPAR1 and p-ERK1/2 in mice of the sham and BCP groups; (b) quantitative analysis of positive expression rate of LPAR1 and p-ERK1/2 proteins in mice of the sham and BCP groups; (c) double-labeling immunofluorescence analysis of LPAR1 and p-ERK1/2 in mice of the sham and BCP groups; (d) the number of p-ERK1/2 positive cells in mice of the sham and BCP groups; n = 10; *p < 0.05 versus the sham group.

BCP, bone cancer pain; ERK, extracellular signal-regulated kinase; LARP1, lysophosphatidic acid receptor 1.