Table 1.
Animal grouping.
| Group | Treatment |
|---|---|
| Sham (n = 14) | Sham operation was performed on the left tibia of C3H/HeJ mice: no 4T1 cells was inoculated and intrathecal injection of normal saline was conducted |
| BCP (n = 14) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with normal saline |
| NC (n = 10) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with nonsense sequence |
| miR-329-mimic (n = 10) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with miR-329 mimic |
| miR-329-mimic + LPAR1-cDNA (n = 10) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with both miR-329 mimic and LPAR1-cDNA viruses |
| LPAR1 shRNA (n = 10) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with LPAR1 shRNA |
| miR-329-mimic + LPAR1 shRNA (n = 10) | The left tibia of BCP mice injected with 4 × 104 4T1 cells (4 μl) and intrathecally injected with both miR-329 mimic and LPAR1 shRNA |
BCP, bone cancer pain; LPAR1, lysophosphatidic acid receptor 1; NC, negative control.