Table 2.
Gene | Accession number | Primer sequence |
---|---|---|
miR-329 | NR_029762.1 | F: 5′-ACACTCCAGCTGGGACTATG-3′ |
R: 5′-TGGTGTCGTGGAGTCG-3′ | ||
LPAR1 | NM_172989.1 | F: 5′-GCTTCTACAATGAGTCTATCGCC-3′ |
R: 5′-TGATGAACACGCAAACAGTGAT-3′ | ||
U6 | NM_001110101.2 | F: 5′-ACCCTGAGAAATACCCTCACAT-3′ |
R: 5′-GACGACTGAGCCCCTGATG-3′ | ||
GAPDH | NM_008084.3 | F: 5′-AGGTCGGTGTGAACGGATTTG-3′ |
R: 5′-GGGGTCGTTGATGGCAACA-3′ |
F, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPAR1, lysophosphatidic acid receptor 1; miR-329, microRNA-329; R, reverse; RT-qPCR, reverse transcription quantitative polymerase chain reaction.