Fig. 3.

Y37A1B.5 is a cytoplasmic protein expressed in hypodermal cells. (A) Schematic representation of the translational reporter construct used in this study, pY37A1B.5::Y37A1B.5::gfp. (E1-E6, exons 1-6) (B) Hypodermal localization of Y37A1B.5::GFP. White box indicates region enlarged for (C). (C) Subcellularly, Y37A1B.5::GFP is localized primarily in the cytoplasm. White arrows indicate nuclei devoid of signal. (D) Few worms display fluorescence in anterior (arrows) or posterior (not shown) intestinal cells. (E) Comparison of RNAi using control (Ctr) or Y37A1B.5-specific (Y37) plasmids on Y37A1B.5::GFP reporter strain. White lines indicate worm shape.