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Prok2 mRNA expression in the MOB is not circadian. Expression in the MOB of Prok2 variants 1 (A) and 2 (B) mRNA were measured in 4 month-old C57Bl/6J mice using RT-qPCR. Mice were maintained under a dark: light cycle of 12 h starting at 7.00 am (ZT0) and samples were taken every 4 h (Zeitgeber time, ZT). Rhythmicity was assessed using Circwave v1.4. Values represent mean with SEM (n = 4–5/group). Data were analyzed using a one way ANOVA, followed by post hoc Bonferroni test.
