Figure S2.

Partial deletion of Prok2 in the MOB affected neither locomotor activity nor energy expenditure. Mice injected in the MOB with the scrambled-shRNA or Prok2-shRNA were placed in calorimetric cages (TSE system, Germany) 3 weeks postsurgery. Locomotor activity (A, B) and energy expenditure (C, D) were measured in fed conditions. Values represent mean with SEM (n = 6/group). The 24h studies (A, C) were analyzed using a two-way ANOVA, followed by post hoc Bonferroni test and the cumulative studies (B, D) were analyzed using an unpaired Student's t-test.