Figure S3.
Partial deletion of Prok2 in the MOB induced changes in respiratory exchange ratio (RER) and fatty acid oxidation in fasted mice. Mice injected in the MOB with the scrambled-shRNA or Prok2-shRNA were placed in calorimetric cages (TSE system, Germany) 3 weeks postsurgery. RER (RER = VCO2/VO2, A-C) and fatty acid oxidation (D-G) were measured in fed conditions (A, B, D, E) or during an overnight fasting (C, F, G). Values represent mean with SEM (n = 6/group). The 24h- and the overnight fasting studies (A, C, D, F, G) were analyzed using 2-way ANOVA followed by post hoc Bonferroni test and the cumulative studies (B, E) were analyzed using an unpaired Student's t-test.