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. 2019 Aug 28;29:182–196. doi: 10.1016/j.molmet.2019.08.016

Figure 3.

Figure 3

Partial deletion of Prok2 in the MOB has no effect on body weight but increases food intake. Representative brain section showing GFP fluorescence in the ventral MOB (1 of the 4 injection sites), 48 h post injection of lentiviral particles expressing Prok2-shRNA. GL: glomerular layer, EPL, external plexiform layer, MCL, mitral cell layer, GCL, granule cell layer. Scale bar = 100 μm (A). Quantification using RT-qPCR of Prok2V1 (B) and Prok2V2 (C) mRNA expression in the MOB, 6 weeks after the injection of a lentiviral vector expressing Prok2-shRNA or a scrambled-shRNA. Body-weight was measured weekly for 4 weeks postsurgery in Prok2-shRNA and scrambled-shRNA-injected mice (D). Total lean mass (E) and total fat mass (F) were measured 3 weeks post lentiviral injection using EchoMRI. Weekly 24 h cumulative food intake (G) was measured in ad libitum fed mice from 1 to 4 weeks post lentiviral injection. Values represent mean with SEM (n = 6–12/group). *, P < 0.05 vs scrambled-shRNA, in an unpaired Student's t-test.