Figure 5.

Partial deletion of Prok2 in the MOB altered glucose metabolism in vivo. Glucose homeostasis studies were performed 4 weeks after injection of Prok2-shRNA or scrambled-shRNA in the MOB. Plasma glucose (A) and insulin (B) were measured during a glucose tolerance test (3  g/kg BW), plasma glucose was measured during an insulin tolerance test (0.75U/kg BW) (C) and glucose uptake was measured after ip insulin (0.75U/kg BW) and 2-deoxy-D-[1–14C] glucose (2DG) (5 μCi) injection, in liver (D), subcutaneous adipose tissue (E), visceral adipose tissue (F), soleus (G), extensor digitorum longus (EDL, H). Values represent mean with SEM (n = 4–6/group), *, P < 0.05 and **, P < 0.01 vs scrambled-shRNA, in two-way ANOVA followed by Bonferroni post hoc test except for glucose uptake that was analyzed using an unpaired Student's t-test.