Table 1.

Partial deletion of Prok2 in the MOB affects the balance of AgRP/POMC neuropeptides expression in the hypothalamus.

A	mRNA relative expression in the MOB
	NPY	AgRP	POMC	CART	AgRP/POMC
Scrambled-shRNA	2.56 ± 0.1	0.08 ± 0.009	0.003 ± 0.009	0.27 ± 0.02	28.7 ± 7.06
Prok2-shRNA	2.2 ± 0.6	0.06 ± 0.003	0.006 ± 0.004	0.27 ± 0.06	17.0 ± 6.6
p value	0.5	0.09	0.7	0.99	0.7
B	mRNA relative expression in the hypothalamus
	NPY	AgRP	POMC	CART	AgRP/POMC
Scrambled-shRNA	0.90 ± 0.3	5.2 ± 3.3	2.2 ± 1.2	4.3 ± 0.7	1.8 ± 0.8
Prok2-shRNA	1.28 ± 0.6	5.8 ± 2.7	0.32 ± 0.15	2.1 ± 0.9	21.9 ± 3.7
p value	0.6	0.9	0.2	0.09	0.002**

Quantification using RT-qPCR of neuropeptide Y (NPY), agouti related peptide (AgRP), proopiomelanocortin (POMC) and cocaine and amphetamine regulated transcript (CART) mRNA expression in the MOB (A) and in the hypothalamus (B) of mice injected with scrambled-shRNA or Prok2-shRNA. Values represent mean with SEM (n = 5–7/group), **, P < 0.01, vs scrambled-shRNA, in an unpaired Student's t-test.