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. 2019 Sep 7;28:101311. doi: 10.1016/j.redox.2019.101311

Fig. 1.

Fig. 1

Doxorubicin inhibits mitochondrial respiration. (A) Platelets from a representative donor are shown with and without incubation with Doxorubicin (Dox) (0–25 μM) for 3 h after which a mitochondrial stress test was performed by first measuring basal OCR, followed by sequential injection of oligomycin (Oligo) (1 μg/ml), FCCP (0.6 μM), antimycin A (AntiA) (10 μM) and 2-deoxyglucose (50 mM). (B) The Dox concentration dependent inhibition of Basal, ATP linked, Maximal and Reserve Capacity. Data is represented as the mean ± SEM for 5–6 technical replicates for this donor. Data from 13 individual donors are shown for (C) basal (basal OCR – AA OCR), (D) ATP-linked (AL) (basal OCR – oligomycin OCR), (E) maximal (FCCP OCR – AA OCR), (F) reserve capacity (RC) (FCCP OCR – basal OCR) (G) proton leak (PL) (oligomycin OCR – AA OCR), calculated and are expressed as mean ± SEM with each dot representing the average data from a single donor, n = 5–6 replicates for each parameter. (H–K) Decrease (% of pre-Dox value) for each donor for basal (H), ATP linked (I) Maximal (J) and Reserve Capacity (K) after treatment with 25 μM Dox.