Fig. 3.

Metabolic separation of features after treatment to Dox in human platelets. (A) Unsupervised hierarchal clustering heatmap indicate that intensity of 166 features which drive the separation between vehicle control and Dox treated groups. Subject sub clusters 1 and 2 are indicated in the vehicle group. (B) PCA plot showing separation of the vehicle control group (shown in green) and BaP exposed group (shown in red), through the 1st and 2nd principal components. (C) Type I Manhattan plot of m/z features plotted against the –Log₁₀P value. Shown in gray are the 2831 features identified after filtering and normalization. 166 features were found to be different between the two groups using the criteria (p < 0.05, FDR < 0.2) as indicated by the blue dotted line. Shown in red were features identified to be increased after Dox exposure (68/166) and in blue features which were decreased (98/166). (D) Type II Manhattan plot using time plotted against –Log₁₀P value. (E) Pathway enrichment analysis of stored human platelets after Dox exposure compared to vehicle control. A total of (7/119) enriched pathways were determined (Filled gray bars indicate significance and the cutoff (p < 0.05) is indicated by the dotted line). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)