Fig. 1.

G6PD activity in different brain regions of young (2–5 months) and aging (12–18 months) G6PD-normal and G6PD-deficient male and female mice. Panel A: Young G6PD wild-type (+/y) normal males compared to G6PD-deficient (def/y) counterparts. Panel B: Young G6PD wild-type (+/+) normal females compared to G6PD-deficient (def/def) counterparts. Panel C: Aging G6PD wild-type (+/y) normal males compared to mutant G6PD-deficient (def/y) counterparts. Panel D: Aging G6PD wild-type (+/+) normal females compared to mutant G6PD-deficient (def/def) counterparts. Panels E-H: Only mutant G6PD-deficient mice. Panel E: Young males compared to young females. Panel F: Aging males compared to aging females. Panel G: Young males compared to aging males. Panel H: Young females compared to aging females. Asterisks indicate significant differences between the G6PD-normal (+/y or +/+) group and the G6PD-deficient (def/y or def/def) group, or between young and aging females, for the same brain region (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). α indicates a difference from cortex (p < 0.0001 in Panels B and D, p < 0.01 in Panel C, p < 0.05 in Panels F – H); β indicates a difference from striatum (p < 0.0001 in cerebellum in Panel B, and in hippocampus in Panel D, p < 0.001 in hippocampus in Panel B, p < 0.05 in cerebellum in Panel D); χ indicates differences from hippocampus (p < 0.0001 in Panel D, p < 0.01 in Panel H). The differences in G6PD activity between age- and sex-matched mice depended upon the brain region analyzed. Statistical analyses were performed using two-way ANOVA, followed by a Bonferroni post-hoc test conducted independently for genotype-dependent differences vs. brain region-dependent differences (Panels A–D), and independently for sex-dependent differences (Panels E–F), or age-dependent differences (Panels G–H) vs. brain region-dependent differences (Panels E− H). For all groups, n = 3. The minimum significance level used throughout was p < 0.05.