Fig. 4.

Effect of G6PD deficiency on double strand breaks. The level of γH2AX, a marker of DNA double-strand breaks in various brain regions, was measured using western blotting. Panels A: Young G6PD wild-type (+/+ or +/y) normal mice compared with mutant G6PD-deficient (def/def or def/y) sex-matched counterparts. Panels B: Aging G6PD wild-type (+/+ or +/y) normal mice compared with mutant G6PD-deficient (def/def or def/y) sex-matched counterparts. Significant differences were found in the striatum of young males as well as the hippocampus of young and aged males and young females. The number of mice for each genotype is shown in parentheses. Statistical analyses were performed using Student's t-test. Asterisks indicate a difference from G6PD-normal mice of the same sex (def/y or +/+) (p < 0.05).