Fig. 5.

Decrease in Purkinje cell (PC) numbers and PC-related calbindin-D-28K expression in aging G6PD-deficient mice. Upper panel: PCs from aging mice (12–18 months) were counted in 10 distinct fields at 40X to give a total of cells for each sample. Statistical analysis was performed using the two-tailed Student's t-test. Asterisks indicate a difference from G6PD-normal controls of the same sex (p < 0.05). Lower panel: Densitometric analysis of calbindin-D-28K protein, a marker for PCs, was performed for cerebellar tissues from G6PD-normal mice (males: +/y; females: +/+) and G6PD-deficient mice (males: def/y; females: +/def, def/def). Statistical analysis was performed using one-way ANOVA. The number of mice for each genotype is shown in parentheses. Asterisks indicate a difference from G6PD-normal wild-type controls (p < 0.05).