Table 1.

Summary of effects of individual Cys-Ser mutations in different assays. Significant up-or downregulations are indicated by arrows, two arrows symbolize effects of more than 50%. Arrows in parentheses indicate trends or qualitative observations. A lack of significant effects is symbolized with a dash. For DCP-Bio1 labeling, ‘uncertain’ indicates large variation of signals in independent assays, which precluded statistical analysis. For detailed description of the different assays and corresponding conclusions, see the text.

Assay		Cysteines
		681	694	695	790	807	828	925	945
HEK293 FLT3ITD	DCP-Bio1 labeling	–	–	↓	↓	–	uncertain	uncertain	uncertain
	Autokinase	(↓)	↓	(↓)	(↑)	(↓)	–	↓	(↓)
	pFLT3ITD	↓	↓↓	–	–	↓↓	–	↓↓	↓
	pSTAT5	↓	↓↓	↓	↑	↓↓	↓	↓↓	↓
	pERK1/2	↓	↓↓	–	–	↓↓	–	↓↓	↓
	Dimerization	(↑)	(↑)	–	(↓)	(↑)	(↓)	–	–
32D FLT3ITD	pFLT3ITD	↓	↓↓	–	–	↓↓	–	↓↓	–
	pSTAT5	↓↓	↓↓	↓↓	↑	↓	–	↓↓	↓↓
	pERK1/2	–	–	–	↑	↓↓	–	↓↓	–
	Cell viability (-IL3)	↓↓	↓↓	↓↓	–	↓↓	–	↓↓	↓↓
	Colony formation	↓↓	↓↓	↓↓	↑	↓↓	–	↓↓	↓↓