Effects of NHBA on ethanol-containing reward-seeking in C57BL/6J mice. (A and B) Experimental scheme. (C) Effects of NHBA (0.03 and 0.1 mg/kg, i.p.) on nose-poking behavior in water (W) presentation state. (D) Nose-poking changes between water (W) and 10% ethanol and 10% sucrose (10E10S) presentation states (n = 5/group). (E) Effects of NHBA (0.1 mg/kg, i.p.) on nose-poking behavior in either water (W) or 10% ethanol (10E) presentation state (n = 8/group). Data represented as mean ± S.E.M. *P < 0.05, (C) comparing vehicle treatment group, (D and E) comparing each different state. (C) One-way ANOVA followed by Bonferroni’s multiple comparisons test, and (D and E) paired Student’s t test.